An article Detoxification of host plant phenolic aglycones by the spruce budworm WOS:000468030100002 published article about GLUTATHIONE-S-TRANSFERASE; HELICOVERPA-ARMIGERA; MIDGUT; RESISTANCE; ECOLOGY; TANNINS; PH in [Donkor, Dominic; Mirzahosseini, Zahra; Despland, Emma] Concordia Univ, Biol Dept, Montreal, PQ, Canada; [Bede, Jacquie] McGill Univ, Dept Plant Sci, Ste Anne De Bellevue, PQ, Canada; [Bauce, Eric] Univ Laval, Dept Sci Bois & Foret, Ste Foy, PQ, Canada in 2019.0, Cited 40.0. The Name is 4′-Hydroxyacetophenone. Through research, I have a further understanding and discovery of 99-93-4. COA of Formula: C8H8O2
This study examines the post-ingestive fate of two host-plant derived small-molecule phenolics (the acetophenones piceol and pungenol) that have previously been shown to be toxic to the outbreaking forest pest, spruce budworm (Choristoneura fumiferana). We test first whether these compounds are transformed during passage through the midgut, and second whether the budworm upregulates activity of the detoxification enzyme glutathione-s-transferase (GST) in response to feeding on these compounds. Insects were reared on either foliage or artificial diet to the fourth instar, when they were transferred individually to one of two treatment diets, either control or phenolic-laced, for approximately 10 days, after which midguts were dissected out and used for Bradford soluble protein and GST enzyme activity analysis. Frass was collected and subjected to HPLC-DAD-MS. HPLC showed that the acetophenones do not autoxidize under midgut pH conditions, but that glucose- and glutathione-conjugates are present in the frass of insects fed the phenolic-laced diet. GST enzyme activity increases in insects fed the phenolic-laced diet, in both neutral pH and alkaline assays. These data show that the spruce budwom exhibits counter-adaptations to plant phenolics similar to those seen in angiosperm feeders, upregulating an important detoxifying enzyme (GST) and partially conjugating these acetophenones prior to elimination, but that these counter-measures are not totally effective at mitigating toxic effects of the ingested compounds in the context of our artifical-diet based laboratory experiment.
COA of Formula: C8H8O2. About 4′-Hydroxyacetophenone, If you have any questions, you can contact Donkor, D; Mirzahosseini, Z; Bede, J; Bauce, E; Despland, E or concate me.
Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles