In 2019.0 BIOCHEMISTRY-US published article about BINUCLEAR METAL CENTER; SP STRAIN TCM1; FLAME RETARDANTS; ACTIVE-SITE; HYDROLYSIS; ENZYME; TOXICITY; PHOSPHORYL; MECHANISM in [Bigley, Andrew N.; Xiang, Dao Feng; Narindoshvili, Tamari; Raushel, Frank M.] Texas A&M Univ, Dept Chem, College Stn, TX 77843 USA; [Burgert, Charlie W.; Hengge, Alvan C.] Utah State Univ, Dept Chem & Biochem, Logan, UT 84322 USA in 2019.0, Cited 38.0. The Name is 4′-Hydroxyacetophenone. Through research, I have a further understanding and discovery of 99-93-4. Recommanded Product: 99-93-4
Organophosphorus flame retardants are stable toxic compounds used in nearly all durable plastic products and are considered major emerging pollutants. The phosphotriesterase from Sphingobium sp. TCM1 (Sb-PTE) is one of the few enzymes known to be able to hydrolyze organophosphorus flame retardants such as triphenyl phosphate and tris(2-chloroethyl) phosphate. The effectiveness of Sb-PTE for the hydrolysis of these organophosphates appears to arise from its ability to hydrolyze unactivated alkyl and phenolic esters from the central phosphorus core. How Sb-PTE is able to catalyze the hydrolysis of the unactivated substituents is not known. To interrogate the catalytic hydrolysis mechanism of Sb-PTE, the pH dependence of the reaction and the effects of changing the solvent viscosity were determined. These experiments were complemented by measurement of the primary and secondary 18-oxygen isotope effects on substrate hydrolysis and a determination of the effects of changing the pK(a) of the leaving group on the magnitude of the rate constants for hydrolysis. Collectively, the results indicated that a single group must be ionized for nucleophilic attack and that a separate general acid is not involved in protonation of the leaving group. The Bronsted analysis and the heavy atom kinetic isotope effects are consistent with an early associative transition state with subsequent proton transfers not being rate limiting. A novel binding mode of the substrate to the binuclear metal center and a catalytic mechanism are proposed to explain the unusual ability of Sb-PTE to hydrolyze unactivated esters from a wide range of organophosphate substrates.
About 4′-Hydroxyacetophenone, If you have any questions, you can contact Bigley, AN; Xiang, DF; Narindoshvili, T; Burgert, CW; Hengge, AC; Raushel, FM or concate me.. Recommanded Product: 99-93-4
Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles