Metabolomics-assisted discovery of a new anticancer GLS-1 inhibitor chemotype from a nortopsentin-inspired library: From phenotype screening to target identification was written by Carbone, Daniela;Vestuto, Vincenzo;Ferraro, Maria Rosalia;Ciaglia, Tania;Pecoraro, Camilla;Sommella, Eduardo;Cascioferro, Stella;Salviati, Emanuela;Novi, Sara;Tecce, Mario Felice;Amodio, Giuseppina;Iraci, Nunzio;Cirrincione, Girolamo;Campiglia, Pietro;Diana, Patrizia;Bertamino, Alessia;Parrino, Barbara;Ostacolo, Carmine. And the article was included in European Journal of Medicinal Chemistry in 2022.Product Details of 90271-86-6 This article mentions the following:
The enzyme glutaminase-1 (GLS-1) has shown a clear and coherent implication in the progression and exacerbation of different aggressive tumors such as glioblastoma, hepatocarcinoma, pancreas, bone, and triple-neg. breast cancer. Few chemotypes are currently available as selective GLS-1 inhibitors, and still, fewer of them are at the clin. stage. In the present paper, starting from a naturally-inspired antitumor compound library, metabolomics has been used to putatively identify the mol. mechanism underlying biol. activity. GLS-1 was identified as a potential target. Biochem. anal. confirmed the hypothesis leading to the identification of a new hit compound acting as a GLS-1 selective inhibitor (IC50 = 3.96 闂?1.05 濠电偞鎸鹃幏?, compared to the GLS-2 isoform (IC50 = 12.90 闂?0.87 濠电偞鎸鹃幏?, with remarkable antitumor potency over different aggressive tumor cell lines. Mol. modeling studies revealed new insight into the drug-target interaction providing robust SAR clues for the rational hit-to-lead development. The approach undertaken underlines the wide potential of metabolomics applied to drug discovery, particularly in target identification and hit discovery following phenotype screening. In the experiment, the researchers used many compounds, for example, 5-Bromo-3-cyanoindole (cas: 90271-86-6Product Details of 90271-86-6).
5-Bromo-3-cyanoindole (cas: 90271-86-6) belongs to indole derivatives. Indole, also called Benzopyrrole, a heterocyclic organic compound occurring in some flower oils, such as jasmine and orange blossom, in coal tar, and in fecal matter. It is used in perfumery and in making tryptophan, an essential amino acid, and indoleacetic acid (heteroauxin), a hormone that promotes the development of roots in plant cuttings.Product Details of 90271-86-6
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Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles