Recently I am researching about MOLECULAR CHAPERONE; ANTICANCER AGENTS; FORCE-FIELD; CANCER; DYNAMICS; DOCKING; DESIGN; OPTIMIZATION; PERFORMANCE; GENERATION, Saw an article supported by the Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt; Natural Sciences and Engineering Research Council (NSERC) of CanadaNatural Sciences and Engineering Research Council of Canada (NSERC) [RGPIN-2017-04233]. Published in ACADEMIC PRESS INC ELSEVIER SCIENCE in SAN DIEGO ,Authors: Mohamady, S; Ismail, MI; Mogheith, SM; Attia, YM; Taylor, SD. The CAS is 99-93-4. Through research, I have a further understanding and discovery of 4′-Hydroxyacetophenone. HPLC of Formula: C8H8O2
Although hepatocellular carcinoma (HCC)-related mortality has increased over the past decades, treatment options are still very limited, underlining the need for developing new therapeutic strategies. The molecular chaperone heat shock protein 90 (Hsp90) plays a key role in post-translational maturation of many oncogenic client proteins that are important for survival and proliferation of cancer cells. Thus, inhibitors of Hsp90 are promising targets for many cancer types. In this study, 15 diarylpyrazole compounds were screened against MCF7 and HepG2 cell lines. Compound 8, which contained a thiophene group, demonstrated the highest antiproliferative activity against HepG2 cells having an IC50, of 0.083 mu M. Four additional diarylpyrazoles, each containing a thiophene group, were prepared and screened for antiproliferative activity. None of these four compounds exhibited superior activity to compound 8 on HepG2 cells. Therefore, compound 8 was selected for further in vitro assays. Cell cycle arrest was observed at the G2 phase in compound 8-treated cells. Compound 8 also caused a 7.7-fold increase in caspase-3. These results confirm the apoptotic effect of compound 8 on HepG2 cells. Moreover, compound 8 inhibited Hsp90 (IC50, = 2.67 +/- 0.18 mu M) in an in vitro assay and caused a 70.8% reduction in Hsp90 levels in a HepG2 cell-based assay. Additionally, compound 8 caused significant reduction in the levels of Hsp90 client proteins (Akt, c-Met, c-Raf, and EGFR) and a 1.57-fold increase in Hsp70. Molecular docking studies were also performed to predict the binding mode of compound 8 and followed by molecular dynamics simulations to give further insights into the binding mode of 8.
HPLC of Formula: C8H8O2. Welcome to talk about 99-93-4, If you have any questions, you can contact Mohamady, S; Ismail, MI; Mogheith, SM; Attia, YM; Taylor, SD or send Email.
Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles