The author of 《Structure-based redesign of a self-sufficient flavin-containing monooxygenase towards indigo production》 were Loncar, Nikola; van Beek, Hugo L.; Fraaije, Marco W.. And the article was published in International Journal of Molecular Sciences in 2019. Product Details of 120-72-9 The author mentioned the following in the article:
Indigo is currently produced by a century-old petrochem.-based process, therefore it is highly attractive to develop a more environmentally benign and efficient biotechnol. process to produce this timeless dye. Flavin-containing monooxygenases (FMOs) are able to oxidize a wide variety of substrates. In this paper we show that the bacterial mFMO can be adapted to improve its ability to convert indole into indigo. The improvement was achieved by a combination of computational and structure-inspired enzyme redesign. We showed that the thermostability and the kcat for indole could be improved 1.5-fold by screening a relatively small number of enzyme mutants. This project not only resulted in an improved biocatalyst but also provided an improved understanding of the structural elements that determine the activity of mFMO and provides hints for further improvement of the monooxygenase as biocatalyst. The experimental part of the paper was very detailed, including the reaction process of 1H-Indole(cas: 120-72-9Product Details of 120-72-9)
1H-Indole(cas: 120-72-9) belongs to indole. Indole, also called Benzopyrrole, a heterocyclic organic compound occurring in some flower oils, such as jasmine and orange blossom, in coal tar, and in fecal matter. It is used in perfumery and in making tryptophan, an essential amino acid, and indoleacetic acid (heteroauxin), a hormone that promotes the development of roots in plant cuttings.Product Details of 120-72-9
Referemce:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles