I found the field of Chemistry very interesting. Saw the article Conversion of 3,6-O-Dimethylfluorescein to Fluorescein-Based Xanthylium Derivative: Characterization and Covalent Attachment to Bovine Serum Albumin published in 2019.0. COA of Formula: C7H8O2, Reprint Addresses Kalhor, HR (corresponding author), Sharif Univ Technol, Chem Dept, Biochem Res Lab, Tehran, Iran.. The CAS is 150-19-6. Through research, I have a further understanding and discovery of m-Methoxyphenol
Xanthene dyes, such as fluorescein and rhodamine, have played crucial rules in biological sciences especially covalent attachment of these organic compounds to biomolecules. 3-O-methylfluorescein and 3,6-O-dimethylfluorescein were synthesized and purified using a facile procedure. 3,6-O-dimethylfluorescein, which lacks fluorescence, was treated with sodium hydroxide and sulfuric acid solutions, giving rise to yellow and green fluorescent compounds, respectively. In both basic and acidic conditions, donor-acceptor structures were formed. The reaction of 3,6-O-dimethylfluorescein with acids, in a reversible manner, emitted green fluorescence, whereas its reaction with hydroxide ion, irreversibly shifted the fluorescence to the yellow region. Using MS spectrometry, the product identity was determined as a xanthylium ion derivative. Stokes shift and quantum yield for xanthylium ion were measured. Various nucleophiles were examined for studying the mechanism of the xanthylium ion in aqueous solutions; the results confirmed that hydroxide ion was mediatory for the emission of the fluorescence. In addition, 3-O-methyl-5(6)-carboxyfluorescein and 3,6-O-dimethyl-5(6)-carboxyfluorescein were synthesized that can potentially be used in bioorthogonal reactions for labeling proteins. The synthesized products were used in covalent attachment to bovine serum albumin (BSA) and their unique fluorescences were detected.
COA of Formula: C7H8O2. Bye, fridends, I hope you can learn more about C7H8O2, If you have any questions, you can browse other blog as well. See you lster.
Reference:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
,Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles