The author of 《Molecular docking and experimental investigation of new indole derivative cyclooxygenase inhibitor to probe its binding mechanism with bovine serum albumin》 were Wani, Tanveer A.; Bakheit, Ahmed H.; Zargar, Seema; Bhat, Mashooq A.; Al-Majed, Abdulrahman A.. And the article was published in Bioorganic Chemistry in 2019. Recommanded Product: 120-72-9 The author mentioned the following in the article:
The indole derivative 2-(5-methoxy-2-methyl-1H-indol-3-yl)-N’-[(E)-(3-nitrophenyl) methylidene]acetohydrazide (IND) was synthesized for its therapeutic potential to inhibit cyclooxygenase (COX)-II. Binding if IND to bovine serum albumin (BSA) was investigated was because most drugs bind to serum albumin in-vivo. Fluorescence, UV-vis spectrophotometry and mol. modeling methodologies were employed for studying the interaction mechanism. The intrinsic fluorescence of BSA was quenched by BSA and the quenching mechanism involved was static quenching. The binding constants between IND and BSA at the three studied temperatures (298, 301 and 306 K) were 1.09 × 105, 4.36 × 104 and 1.23 × 104 L mol-1 resp. The most likely site for binding IND to BSA was Site I (subdomain IIA). The anal. of thermodn. parameter revealed the involvement of hydrogen bonding and van der Waals forces in the IND-BSA interaction. Synchronous fluorescence spectroscopic (SFS) and UV-vis spectrophotometric studies suggested conformational change in BSA mol. post interaction to IND. Mol. docking and the exptl. results corroborated one another. The study can prove as an insight for future IND drug development.1H-Indole(cas: 120-72-9Recommanded Product: 120-72-9) was used in this study.
1H-Indole(cas: 120-72-9) belongs to indole. Indole, also called Benzopyrrole, a heterocyclic organic compound occurring in some flower oils, such as jasmine and orange blossom, in coal tar, and in fecal matter. It is used in perfumery and in making tryptophan, an essential amino acid, and indoleacetic acid (heteroauxin), a hormone that promotes the development of roots in plant cuttings.Recommanded Product: 120-72-9
Referemce:
Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles