《Indole contributes to tetracycline resistance via the outer membrane protein OmpN in Vibrio splendidus》 was written by Zhang, Shanshan; Shao, Yina; Zhao, Xuelin; Li, Chenghua; Guo, Ming; Lv, Zhimeng; Zhang, Weiwei. SDS of cas: 120-72-9This research focused onVibrio indole tetracycline resistance outer membrane protein OmpN; Indole; Outer membrane protein; Tetracycline resistance; Vibrio splendidus. The article conveys some information:
As an interspecies and interkingdom signaling mol., indole has recently received attention for its diverse effects on the physiol. of both bacteria and hosts. In this study, indole increased the tetracycline resistance of Vibrio splendidus. The minimal inhibitory concentration of tetracycline was 10μg/mL, and the OD600 of V. splendidus decreased by 94.5% in the presence of 20μg/mL tetracycline; however, the OD600 of V. splendidus with a mixture of 20μg/mL tetracycline and 125μM indole was 10- or 4.5-fold higher than that with only 20μg/mL tetracycline at different time points. The percentage of cells resistant to tetracycline was 600-fold higher in culture with an OD600 of approx. 2.0 than that in culture with an OD600 of 0.5, which also meant that level of indole was correlated to tetracycline resistance of V. splendidus. Furthermore, one differentially expressed protein, which was identified as outer membrane porin OmpN using SDS-PAGE combined with MALDI-TOF/TOF MS, was upregulated. Consequently, expression of the ompN gene in the presence of either tetracycline or indole and simultaneously in the presence of indole and tetracycline was upregulated by 1.8-, 2.54-, and 6.01-fold, resp., compared to the control samples. The combined results demonstrated that indole enhanced the tetracycline resistance of V. splendidus, and this resistance was probably due to upregulation of the outer membrane porin OmpN. The experimental process involved the reaction of 1H-Indole(cas: 120-72-9SDS of cas: 120-72-9)
1H-Indole(cas: 120-72-9) belongs to indole. Indole, also called Benzopyrrole, a heterocyclic organic compound occurring in some flower oils, such as jasmine and orange blossom, in coal tar, and in fecal matter. It is used in perfumery and in making tryptophan, an essential amino acid, and indoleacetic acid (heteroauxin), a hormone that promotes the development of roots in plant cuttings.SDS of cas: 120-72-9
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Indole alkaloid derivatives as building blocks of natural products from Bacillus thuringiensis and Bacillus velezensis and their antibacterial and antifungal activity study,
Preparation of Indole Containing Building Blocks for the Regiospecific Construction of Indole Appended Pyrazoles and Pyrroles